Optimation of tempe liquid waste usage for growth performances and feed uptake on Oreochromis niloticus

Soni Andriawan, David Hermawan, Shafira Rahmania

Abstract


Tempe liquid waste (TLW) contains nutrients such as fat, protein, carbohydrates, nitrogen, phosphate, and potassium that could be used in fish rearing. Moreover, it has lactic acid bacteria (LAB) belonging to gram-positive microorganisms, which could decompose organic and inorganic matter in water. Therefore, this study aimed to determine whether TLW could have the potential to enhance Tilapia (Oreochromis niloticus) growth and improve feed efficiency. The study used 180 fish (6.04 ± 0.13 g) reared in twelve aquariums and TLW was obtained from boiling soybeans during the tempe production process in the village of Sanan, Malang. The research design was Completely Randomized Design (CRD), which involved four treatments, T0 (no TLW), T1 (3%), T2 (5%), and T3 (7%) with three replications. The result showed that T2 resulted in the highest growth rate (11.22 ± 0.29 g), specific growth rate (2.07 ± 0.32% / day), feed conversion rate (1.54 ± 0.16), feed efficiency (56.02 ± 1.24%) and survival rate (91.11 ± 0.58%) compared to others. Nonetheless, there was no difference in the absolute length (Lm) parameter at the end of the research. The research results also found that water quality remained favourable for supporting tilapia cultivation under all observed conditions, except for the oxygen levels in the control group, which fell below the standard (<3 mg L-1). Finally, the present study concluded that 5% of TLW could be the best; in contrast, the higher concentration of TLW decreased O. niloticus growth performance, feed efficiency, and survival rate.

Keywords: fish development, tilapia, wastewater, water qualities


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References


Abdel-Tawwab, M., Hagras, A. E., Elbaghdady, H. A. M., Monier, M. N. 2015. Effects of dissolved oxygen and fish size on Nile tilapia, Oreochromis niloticus (L.): growth performance, whole-body composition, and innate immunity. Aquaculture International 23: 1261-1274. https://doi.org/10.1007/s10499-015-9882-y

Ahnan-Winarno, A. D., Cordeiro, L., Winarno, F. G., Gibbons, J., Xiao, H. 2021. Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability. Comprehensive Reviews in Food Science and Food Safety 20: 1717-1767. https://doi.org/https://doi.org/10.1111/1541-4337.12710

Alavi, S., Mazumdar, S. D., Taylor, J. R. N. (2019). Chapter 10 - Modern Convenient Sorghum and Millet Food, Beverage and Animal Feed Products, and Their Technologies. In J. R. N. Taylor & K. G. Duodu (Eds.), Sorghum and Millets (Second Edition) (pp. 293-329). AACC International Press. https://doi.org/https://doi.org/10.1016/B978-0-12-811527-5.00010-1

Amaliah, Z., Bahri, S., Amelia, P. 2018. Isolation and characterization of lactic acid bacteria from soybean soaking liquid waste. Jurnal Fitofarmaka Indonesia 5: 253-257.

Anggraini, F. 2019. [Utilization of liquid waste and solid waste at the Sanan industrial center, Malang City] Universitas Brawijaya]. Indonesia.

Arvanitoyannis, I. S., Tserkezou, P. 2014. Irradiation of Fish and Seafood. Seafood Processing: Technology, Quality and Safety 83-127.

Astuti, M., Meliala, A., Dalais, F. S., Wahlqvist, M. L. 2000. Tempe, a nutritious and healthy food from Indonesia. Asia Pacific Journal of Clinical Nutrition 9: 322-325. https://doi.org/https://doi.org/10.1046/j.1440-6047.2000.00176.x

Bujna, E., Farkas, N. A., Tran, A. M., Sao Dam, M., Nguyen, Q. D. 2018. Lactic acid fermentation of apricot juice by mono-and mixed cultures of probiotic Lactobacillus and Bifidobacterium strains. Food science biotechnology 27: 547-554.

Cokrowati, N. 2021. Pengaruh luas penampang sistem resirkulasi yang berbeda terhadap kualitas air pada pemeliharaan ikan nila (Oreochromis niloticus). Jurnal Perikanan dan Kelautan 26: 85-93.

Dangeubun, J. L., Serang, A. M., Syahailatua, D. Y. 2019. Growth, food efficiency, and survivorship of several fish species treated with artificial feed enriched with Alstonia acuminata. Aquaculture, Aquarium, Conservation Legislation 12: 832-839.

de Verdal, H., Komen, H., Quillet, E., Chatain, B., Allal, F., Benzie, J. A. H., Vandeputte, M. 2018. Improving feed efficiency in fish using selective breeding: a review. Reviews in Aquaculture 10: 833-851. https://doi.org/https://doi.org/10.1111/raq.12202

Efendi, H. 2003. [Water quality review]. Kanisius, Yogyakarta. 257 pp.

El-Sayed, A.-F. M. 2019. Tilapia culture 2nd. Academic Press, Egypt.

Eldridge, W. H., Sweeney, B. W., Law, J. M. 2015. Fish growth, physiological stress, and tissue condition in response to rate of temperature change during cool or warm diel thermal cycles. Canadian Journal of Fisheries and Aquatic Sciences 72: 1527-1537. https://doi.org/10.1139/cjfas-2014-0350

Fradina, I. T., Latuconsina, H. 2022. Manajemen Pemberian Pakan pada Induk dan Benih Ikan Nila (Oreochromis niloticus) di Instalasi Perikanan Budidaya, Kepanjen-Kabupaten Malang. JUSTE (Journal of Science and Technology) 3: 39-45.

Gu, D. E., Yu, F. D., Yang, Y. X., Xu, M., Wei, H., Luo, D., Mu, X. D., Hu, Y. C. 2019. Tilapia fisheries in Guangdong Province, China: Socio?economic benefits, and threats on native ecosystems and economics. Fisheries Management Ecology 26: 97-107.

Guo, H., Lin, W., Yang, L., Qiu, Y., Kuang, Y., Yang, H., Zhang, C., Li, L., Li, D., Tang, R. 2021. Sub?chronic exposure to ammonia inhibits the growth of juvenile Wuchang bream (Megalobrama amblycephala) mainly by downregulation of growth hormone/insulin?like growth factor axis. Environmental Toxicology 36: 1195-1205.

Guo, T., Xu, S., Yu, J., Liu, Q., Gao, G., Li, J., Wang, Y. 2022. Elevated CO2 reduces juvenile Scophthalmus maximus growth and liver function in a recirculating aquaculture system. Authorea Preprints.

Hadioetomo, R. S. 1990. [Basic microbiology in practice: basic laboratory techniques and procedures]. PT Gramedia, Indonesia.

Harahap, S. 2013. [Water pollution due to high levels of ammonia from the tempe industrial wastewater]. Jurnal Akuatika 4: 183-194. http://jurnal.unpad.ac.id/akuatika/article/view/3142

Hartini, S., Letsoin, F., Kristijanto, A. I. 2018. Productive liquid fertilizer from liquid waste tempe industry as revealed by various EM4 concentration. IOP Conference Series: Materials Science and Engineering 349: 012059-012068. https://doi.org/10.1088/1757-899x/349/1/012059

Hertika, A. M. S., Kilawati, Y. K., Fajriani, S., Rosyidah, Z., Anggraini, D., Iswati, I., Wasti, D. R. N., Ni, A. M. N. m. M., Afandy, I. A., Sembodo, P. A. 2021. Pendampingan Kegiatan Monitoring Kualitas Air Pada Pembudidaya Udang Vaname di Kabupaten Probolinggo. Journal of Innovation and Applied Technology 7: 1145-1153.

Heryanto, H. 2020. Utilization of tempeh liquid waste in cultural water media for growth and feed utilization efficiency on Nile tilapia (Oreochromis niloticus) Universitas Muhammadiyah Malang]. Indonesia. 21 pp.

Kamaruddin, M. A., Yusoff, M. S., Aziz, H. A., Basri, N. K. 2013. Removal of COD, ammoniacal nitrogen and colour from stabilized landfill leachate by anaerobic organism. Applied Water Science 3: 359-366. https://doi.org/10.1007/s13201-013-0086-1

Lawrence, J., Fenaux, L., Corre, M. C., Lawrence, A. (2020). The effect of quantity and quality of prepared diets on production in Paracentrotus lividus (Echinodermata: Echinoidea). In Echinoderm research 1991 (pp. 107-110). CRC Press.

Madenjian, C. P., Rediske, R. R., Krabbenhoft, D. P., Stapanian, M. A., Chernyak, S. M., O’Keefe, J. P. 2016. Sex differences in contaminant concentrations of fish: a synthesis. Biology of Sex Differences 7: 42. https://doi.org/10.1186/s13293-016-0090-x

Mahfoedz, M. M. 2014. Laporan tugas akhir identifikasi limbah cair di industri tempe murni muchlar Bantul, Yogyakarta [Yogyakarta]: Universitas Gadjah Mada].

Mallya, Y. J. 2007. The effects of dissolved oxygen on fish growth in aquaculture. The United Nations University Fisheries Training Programme, Final Project.

Markowiak, P., ?li?ewska, K. 2017. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 9: 1021-1031. https://doi.org/10.3390/nu9091021

Montoya?Camacho, N., Marquez?Ríos, E., Castillo?Yáñez, F. J., Cárdenas López, J. L., López?Elías, J. A., Ruíz?Cruz, S., Jiménez?Ruíz, E. I., Rivas?Vega, M. E., Ocaño?Higuera, V. M. 2019. Advances in the use of alternative protein sources for tilapia feeding. Reviews in Aquaculture 11: 515-526.

Mulqan, M., Rahimi, E., Afdhal, S., Dewiyanti, I. 2017. Growth and survival of agile tilapia (Oreochromis niloticus) seeds in aquaponic systems with different plant species [B.Sc Thesis, Syiah Kuala University]. Malaysia. pp. 68.

Ndubuisi, U. C., Chimezie, A. J., Chinedu, U. C., Chikwem, I. C., Alexander, U. 2015. Effect of pH on the growth performance and survival rate of Clarias gariepinus fry. International Journal of Research in Biosciences 4: 14-20.

Nesje, J. 2018. Impacts of organic matter removal efficiency on the microbial carrying capacity and stability of land-based recirculating aquaculture systems NTNU].

Novik, G., Meerovskaya, O., Savich, V. 2017. Waste degradation and utilization by lactic acid bacteria: use of lactic acid bacteria in production of food additives, bioenergy and biogas. Food Additives 5: 105-146.

Nursyam, H. 2017. Aquatic environmental quality survey on turtle conservation at "Bajulmati" beach, Malang district, East Java, Indonesia. Ponte Academic Journal 73: 46-55.

Omasaki, S. K., Janssen, K., Besson, M., Komen, H. 2017. Economic values of growth rate, feed intake, feed conversion ratio, mortality and uniformity for Nile tilapia. Aquaculture 481: 124-132. https://doi.org/https://doi.org/10.1016/j.aquaculture.2017.04.013

Paladhi, A. G., Joshi, J. T., George, A., Manohar, M., Vallinayagam, S., Malik, J. A. (2022). Lipase and lactic acid bacteria for biodegradation and bioremediation. In Microbes and Microbial Biotechnology for Green Remediation (pp. 265-286). Elsevier.

Parra, J. E. G., Baldisserotto, B. (2019). Effect of water pH and hardness on survival and growth of freshwater teleosts. In Fish osmoregulation (pp. 135-150). CRC Press.

Popma, T., Masser, M. 1999. Tilapia life history and biology. SRAC Publication 1-4.

Prasetio, J., Widyastuti, S. 2020. [Liquid organic fertilizer from Tempe industrial waste]. WAKTU: Jurnal Teknik UNIPA 18: 22-32.

Putra, O., Nursantoso, W., Yunanda, S. 2018. Processing and Utilization of Tempe Liquid Waste to be Organic Fertilizer. Kontribusia 1: 22-32.

Quinto, E. J., Jiménez, P., Caro, I., Tejero, J., Mateo, J., Girbés, T. 2014. Probiotic lactic acid bacteria: a review. Food Nutrition Sciences 5: 1765-1775.

Rajabiesterabadi, H., Yousefi, M., Hoseini, S. M. 2020. Enhanced haematological and immune responses in common carp Cyprinus carpio fed with olive leaf extract-supplemented diets and subjected to ambient ammonia. Aquaculture Nutrition 26: 763-771. https://doi.org/https://doi.org/10.1111/anu.13035

Rieuwpassa, F. 2022. Extraction and characterization of fish protein concentrate from Tilapia. Food Research 6: 92-99.

Ringø, E., Doan, H. V., Lee, S., Song, S. K. 2020. Lactic Acid Bacteria in Shellfish: Possibilities and Challenges. Reviews in Fisheries Science & Aquaculture 28: 139-169. https://doi.org/10.1080/23308249.2019.1683151

Riva, A. F., Sumiyati, S., Wardana, I. W. 2014. [Decreasing levels of COD and TSS in tempeh-making industrial waste with biofilm technology using a combination of bio-filter media with bioball and shell waste] [B.Sc Thesis, Diponegoro University]. Indonesia. pp. 60.

Salsabila, M., Suprapto, H. 2018. [Tilapia (Oreochromis niloticus) rearing technique at the pandaan freshwater aquaculture installation, East Java]. Journal of Aquaculture Fish Health 7: 118-123.

Samsundari, S., Wirawan, G. A. 2013. [Analysis of the application of biofilters in a recirculation system on the quality of water quality of eel fish farming (Anguilla bicolor)]. Jurnal gamma 8: 86-97.

Santosa, E. A., Retnaningrum, E. 2020. [Phenotypic characterization and antimicrobial activity of lactic acid bacteria from Tempe production waste]. Jurnal Sains Dasar 9: 1-10.

Sari, D., Rahmawati, A. 2020. [Analysis of the liquid waste content of tempeh, boiled water and soybean soaking water]. Jurnal Ilmiah Kesehatan Media Husada 9: 36-41.

Schindler Wildhaber, Y., Liechti, R., Alewell, C. 2012. Organic matter dynamics and stable isotope signature as tracers of the sources of suspended sediment. Biogeosciences 9: 1985-1996.

Seesuriyachan, P., Kuntiya, A., Sasaki, K., Techapun, C. 2009. Biocoagulation of dairy wastewater by Lactobacillus casei TISTR 1500 for protein recovery using micro-aerobic sequencing batch reactor (micro-aerobic SBR). Process Biochemistry 44: 406-411. https://doi.org/https://doi.org/10.1016/j.procbio.2008.12.006

Setiawati, D. A., Putra, G. M. D., Khalil, F. I., Zulfikar, W., Hirjani, H. 2019. Aplikasi Kombinasi Filter Bertingkat Untuk Pengolahan Limbah Cair Industri Tempe Di Kelurahan Kekalik Jaya Kota Mataram. Abdi Insani 6: 13-24.

Shah, N., Patel, A. 2013. Lactic acid bacteria in the treatment of dairy waste and formation of by-products-a promising approach. Indian Food Industry Mag. 32: 45-49.

Sheikh, S., Baig, M. A., Ali, N., Khan, N. 2017. Hydrogen sulfide gas poisoning in fish garbage room: A report of a fisherman. Journal of Pakistan Medical Association 67: 1097.

Soomro, A., Masud, T., Anwaar, K. 2002. Role of lactic acid bacteria (LAB) in food preservation and human health-a review. Pakistan Journal of Nutrition 1: 20-24.

Supinah, P., Setiawan, W. F., Mulya, S. P. 2020. Sosialisasi Pemanfaatan Limbah Tempe Sebagai Pupuk Organik Cair untuk Pengelolaan Berkelanjutan di Desa Kuripan Kertoharjo. Jurnal Pusat Inovasi Masyarakat 2: 642?646-642?646.

Tsai, H. Y., Hamilton, A., Guy, D. R., Tinch, A. E., Bishop, S. C., Houston, R. D. 2015. The genetic architecture of growth and fillet traits in farmed Atlantic salmon (Salmo salar). BMC Genetics 16: 51-62. https://doi.org/10.1186/s12863-015-0215-y

Urbina, M. A., Glover, C. N. 2015. Effect of salinity on osmoregulation, metabolism and nitrogen excretion in the amphidromous fish, inanga (Galaxias maculatus). Journal of Experimental Marine Biology and Ecology 473: 7-15. https://doi.org/https://doi.org/10.1016/j.jembe.2015.07.014

Utami, T. S., Arbianti, R., Mariana, M., Karina, N. D., Leondo, V. 2018. The effects of biofilm and selective mixed culture on the electricity outputs and wastewater quality of tempe liquid waste based microbial fuel cell. Reaktor 18: 84-91.

Wijaya, S. 2019. Indonesian food culture mapping: a starter contribution to promote Indonesian culinary tourism. Journal of Ethnic Foods 6: 9-19. https://doi.org/10.1186/s42779-019-0009-3

Workagegn, K., Dadebo, E., Tilahun, G., Ashagre, T. 2014. Growth performance of the Nile Tilapia (Oreochromis niloticus L.) fed different types of diets formulated from varieties of feed ingredients. J. Aquac. Res. Development 5: 235-239. https://doi.org/10.4172/2155-9546.1000235

Xie, H., LüXiao, Zhou, J., Shi, C., Li, Y., Duan, T., Li, G., Luo, Y. 2017. Effects of acute temperature change and temperature acclimation on the respiratory metabolism of the snakehead. Turkish Journal of Fisheries and Aquatic Sciences 17: 535-542.

Yu, J., Wang, Y., Xiao, Y., Li, X., Zhou, L., Wang, Y., Du, T., Ma, X., Li, J. 2021. Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems. Ecotoxicology and Environmental Safety 208: 111617.

Zhang, M., Li, M., Wang, R., Qian, Y. 2018. Effects of acute ammonia toxicity on oxidative stress, immune response and apoptosis of juvenile yellow catfish Pelteobagrus fulvidraco and the mitigation of exogenous taurine. Fish & Shellfish Immunology 79: 313-320. https://doi.org/https://doi.org/10.1016/j.fsi.2018.05.036

Zhang, T., Sun, R., Ding, M., Tao, L., Liu, L., Tao, N., Wang, X., Zhong, J. 2020. Effect of extraction methods on the structural characteristics, functional properties, and emulsion stabilization ability of Tilapia skin gelatins. Food chemistry 328: 127114. https://doi.org/https://doi.org/10.1016/j.foodchem.2020.127114

Zhang, W., Xia, S., Zhu, J., Miao, L., Ren, M., Lin, Y., Ge, X., Sun, S. 2019. Growth performance, physiological response and histology changes of juvenile blunt snout bream, Megalobrama amblycephala exposed to chronic ammonia. Aquaculture 506: 424-436. https://doi.org/https://doi.org/10.1016/j.aquaculture.2019.03.072

???, ???, ???. 2009. Preliminary Study of the Effects of CO2 on the Survival and Growth of Olive Flounder (Paralichthys olivaceus) Juveniles. Fisheries and Aquatic Sciences 12: 350-353.




DOI: http://dx.doi.org/10.20884/1.oa.2024.20.1.1076

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