The Effect of Stocking Density on Survival and Growth of Tiger Shrimp (Penaeus monodon) Cultivated with Seaweed (Gracilaria sp.) in Traditional Pond
Abstract
In order to overcome the decline of pond carrying capacity due to intensification, polyculture of Penaeus monodon with Gracilaria sp. can be implemented to allow sustainability. This study aimed to evaluate the effect of shrimp densities in a polyculture system with Gracilaria sp. on the shrimp’s survival rate and growth. The research design used in this study was Randomized Block Design (RBD) in triplicate with 3 treatments of stocking density (2, 3, and 4 ind/m2). This research was carried out for 90 days in extensive ponds with 0% use of artificial feed. At the end of the study, the results showed that different stocking density applied had no significant effect (p>0.05) on growth performance. Nonetheless, shrimp at a stocking density of 2 ind/m2 had the highest survival rate (30.30±1.48%) compared to other treatments. Higher shrimp density causes competition for space, food, and oxygen which leads to stress resulting in suppressed growth of shrimp. However, Gracilaria integration in shrimp polyculture effectively reduces nutrient loads and stabilize water quality (temperature, pH, salinity, and DO), leading to improved shrimp health and a decreased need for chemical inputs, offering a practical, eco-friendly solution for maintaining shrimp growth in such a denser environment.
Keywords: algae, aquaculture, bioremediation, coastal, integrated
Full Text:
PDFReferences
Abdussamad, E. M., and Thampy, D. M. 1994. Cannibalism in the tiger shrimp Penaeus monodon Fabricius in nursery rearing phase. Journal of Aquaculture in the Tropics, 9(1), 67-75.
Abu Hena M. K., Hishamuddin O., Misri K., Abdullah F., and Loo K. K., 2006 Benthic faunal composition of Penaeus monodon Fabricius culture pond in west coast of peninsular Malaysia. Journal of Biological Sciences, 4(5):631-636.
Abualreesh MH. 2021. Biodiversity and contribution of natural foods in tiger shrimp (Penaeus monodon) aquaculture pond system: a review. Aquac Aquar Conserv Legis Jounal, 14(3):1715–1726.
AftabUddin, S., Siddique, M. A. M., Sein, A., Dey, P. K., Rashed-Un-Nabi, M., and Haque, M. A. 2020. First use of biofloc technology for Penaeus monodon culture in Bangladesh: Effects of stocking density on growth performance of shrimp, water quality and bacterial growth. Aquaculture Reports, 18, 100518.
Amalia, R., Rejeki, S., Widowati, L. L., and Ariyati, R. W. 2022. The growth of tiger shrimp (Penaeus monodon) and its dynamics of water quality in integrated culture. Biodiversitas Journal of Biological Diversity, 23(1).
Anand, P. S. S, Balasubramanian, C. P., Christina, L., Kumar, S., Biswas, G., De, D., Ghoshal, T. K., and Vijayan, K. K. (2019). Substrate based black tiger shrimp, Penaeus monodon culture: Stocking density, aeration and their effect on growth performance, water quality and periphyton development. Aquaculture, 507, 411-418.
Andayani S, Yuwanita R, and Izzah N. 2016. Biofilter application using seaweed (Gracillaria verucosa) to increase production of Vannameii shrimp in traditional pond district Bangil-Pasuruan. Journal of Life Sci 3:16–22.
Anh, N.T.N. and Ngan, P.T.T. 2017. Effects of different feeding rates on water quality and feed efficiency of black tiger shrimp (Penaeus monodon) co-cultured with sea grape (Caulerpa lentillifera). Vietnam. J. Agric. Sci, 10, 119–124.
Anh, N. T. N., Vinh, N. H., An, B. N. T., Lan, L. M., and Hai, T. N. 2020. Polyculture culture of black tiger shrimp Penaeus monodon and red seaweed Gracilaria tenuistipitata under different densities: Effects on water quality, post-larvae performance and their resistance against Vibrio parahaemolyticus. Journal of Applied Phycology, 32(6), 4333-4345.
Anh, N. T. N., Vinh, N. H., Doan, D. T., Lan, L. M., Kurihara, A., and Hai, T. N. 2022. Co-culture of red seaweed Gracilaria tenuistipitata and black tiger shrimp Penaeus monodon in an improved extensive pond at various stocking densities with partially reduced feed rations: a pilot-scale study. Journal of Applied Phycology, 34(2), 1109-1121.
Anh, N. T. N., Nam, T. N. H., Lan, L. M., Kurihara, A., & Hai, T. N. 2024. Polyculture of black tiger shrimp Penaeus monodon, mud crab Scylla paramamosain and red seaweed Gracilaria tenuistipitata, in improved extensive ponds in the Mekong delta, Vietnam. Aquaculture International, 32(2): 1975-2003.
Arambul-Muñoz, E., Ponce-Palafox, J. T., Claro De Los Santos, R., AragónNoriega, E. A., Rodríguez-Domínguez, G., and Castillo-Vargasmachuca, S. G. 2019. Influence of Stocking Density on Production and Water Quality of a Photoheterotrophic Intensive System of White Shrimp (Penaeus vannamei) In Circular Lined Grow-Out Ponds, with minimal water replacement. Journal of Aquatic Research., 47(3): 449-455.
Ariyati, R.W., Sya’rani, L, and Arini, E. 2007.The Suitability Analysis of Karimunjawa and Kemujan Island Territory for Seaweed Culture Site Using Geographical Information System.Jurnal Pasir Laut, 3(1): 27-45.
Arnold, S. J., Coman, F. E., Jackson, C. J., and Groves, S. A. 2009. High-intensity, zero water-exchange production of juvenile tiger shrimp, Penaeus monodon: an evaluation of artificial substrates and stocking density. Aquaculture, 293(1-2), 42-48.
BBPBAP. 2017. Petunjuk Teknis Budidaya Udang Windu Pola Sederhana Melalui Penerapan BMPs (Best Management Practices). Balai Besar Perikanan Budidaya Air Payau Jepara. Jepara, 37p.
Boyd C. E, and Phu T. Q. 2018. Environmental factors and acute hepatopancreatic necrosis disease (AHPND) in shrimp ponds in Vietnam: practices for reducing risks. Asian Fish Sci 31S:121–136.
Choeronawati, A. I., S. B. Prayitno, and Haeruddin Haeruddin. 2019. Feasibility study of aquaculture ponds in the coastal area of Purworejo District. Jurnal Ilmu dan Teknologi Kelautan Tropis, 11(1), 191-204.
Edwards, P. 2015. Aquaculture environment interactions: past, present and likely future trends. Journal of Aquaculture, 447, 2-14.
FAO. 2022. The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome, FAO. Available at https://doi.org/10.4060/cc0461en. Accessed on 26th September 2023.
Gunalan, B., Soundarapandian, P., and Dinakaran, G. K. 2010. Effect of different stocking densities on the MBV infected seeds of black tiger shrimp, Penaeus monodon (Fabricius). Asian Journal of Agricultural Sciences, 2(1), 5-8.
Hendrajat, E. A., and Andi S., 2018. Dominant water quality variables affecting plankton abundance in traditional brackish water ponds of tiger shrimp (Penaeus monodon Fabr.) in Pasuruan Regency, East Java Province. Omni-Akuatika, 14(1), 77-86.
Diatin, I., Effendi, I., and Taufik, M. A. 2020. The production function and profitability analysis of Gracilaria sp. seaweed polyculture with milkfish (Chanos chanos) and black tiger shrimp (Penaeus monodon). Biodiversitas Journal of Biological Diversity, 21(10), 4747-4754.
Khatimah, K., Sompa, A., Haerunnisa, H., and Zaenab, S., 2023. The Potential of Seaweed Gracilaria sp. as An Organic Waste Bioremediation Agent. International Journal of Applied Biology, 7(1), 15-25.
Lemonnier, H., Bernard, E., Boglio, E., Goarant, C., and Cochard, J. C. 2004. Influence of sediment characteristics on shrimp physiology: pH as principal effect. Aquaculture, 240(1-4), 297-312.
Martínez?Curci, N. S., Fierro, P., & Navedo, J. G. (2023). Does experimental seaweed cultivation affect benthic communities and shorebirds? Applications for extensive aquaculture. Ecological Applications, 33(3), e2799.
Millard, R. S., Ellis, R. P., Bateman, K. S., Bickley, L. K., Tyler, C. R., van Aerle, R., and Santos, E. M. 2020. How do abiotic environmental conditions influence shrimp susceptibility to disease? A critical analysis focussed on White Spot Disease. Journal of invertebrate pathology, 186, 107369.
Peraturan Pemerintah Republik Indonesia. 2001. Pengelolaan Kualitas Air dan Pengendalian Pencemaran sehubungan dengan klasifikasi dan kriteria baku mutu air limbah kelas II. No 82 Tahun 2001.
Pescod, N. B. 1973. Investigation of Rational Effluent and Stream For Tropical Countries. AIT. Bangkok.
Rejeki, S., Ariyati. R. W., Widowati, L. L. and Bosma, R. H. 2018. The effect of three cultivation methods and two seedling types on growth, agar content and gel strength of Gracilaria verrucosa. Egyptian Journal of Aquatic Research. 44 : 65-70.
Rodrigues, V., Estrany, J., Ranzini, M., de Cicco, V., Martín-Benito, J. M. T., Hedo, J., & Lucas-Borja, M. E. 2017. Effects of land use and seasonality on stream water quality in a small tropical catchment: The headwater of Córrego Água Limpa, São Paulo (Brazil). Science of the total environment, 622: 1553-1561.
Roleda MY, Hurd CL. 2019. Seaweed nutrient physiology: application of concepts to aquaculture and bioremediation. Journal of Phycologia. 58(5):552–562
Schabhüttl, S., Hingsamer, P., Weigelhofer, G., Hein, T., Weigert, A., and Striebel, M. 2013. Temperature and species richness effects in phytoplankton communities. Oecologia, 171, 527-536.
Sivaraman, I., Krishnan, M., and Radhakrishnan, K. 2018. Better Management Practices for sustainable small-scale shrimp farming. Journal of Cleaner Production, 214, 559-572.
SNI. Standar Nasional Indonesia. 2010. Produksi Rumput Laut Gracilaria (Gracilaria verrucosa) Dengan Metode Tebar di Tambak Secara Polikultur. BSNI 7578:2010.
SNI. Standar Nasional Indonesia. 2021. Udang windu (Penaeus monodon, Fabricius 1798) Bagian 4: Pembesaran Calon Induk di Tambak . BSNI 8554-4:2021.
Sun, S., Fu, H., Gu, Z., Zhu, J. 2016. Effects of stocking density on the individual growth and differentiation of the oriental river prawn Macrobrachium nipponense (de Haan, 1849) (Caridea:Palaemonidae). J Crustacean Biol 36:769–775.
Taslihan, A., Callinan, R., Torribio, J. A., Sumiarto, B., and Nitimulyo, K. H. 2015. Cluster Model for Extensive Giant Tiger Shrimp (Penaeus monodon Fab.) to Prevent Transmission of White Spot Syndrome virus. Indonesian Aquaculture Journal., 10(1): 65-70.
Trianti, C. M., and Ratih. I.A., 2020. The Utilization of Gracilaria verrucosa as shrimp ponds wastewater biofilter. In E3S Web of Conferences (Vol. 147, p. 02023). EDP Sciences.
Tsai, S.J dan J.C. Chen. 2002. Acute toxicity of nitrate on Penaeus monodon juveniles at different salinity levels. Aquaculture. CCXIII: 163- 170.
Varghese, B., Laxminarayana, A., and Daniel, S. D. 2020. Influence of salinity on the growth and body composition of juvenile Tiger Shrimp, Penaeus monodon. Journal of Aquatic Biology and Fisheries, 8, 47-51.
Widowati, L. L., Elfitasari, T., Prayitno, S. B., Rejeki, S., Purnomo, P. W., Ariyati, R. W., and Bosma, R. H. 2020. The Role of Seaweed (Gracilaria verucosa) In Co-Cultivation With Tiger Shrimp (Penaeus Monodon) as An Ecological Intensification. Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology., 16(3): 179-186.
WWF. 2014. BMP Budidaya Udang Windu (Penaeus monodon) Tambak Tradisional dan Semi Intensif. Versi 2. Jakarta Selatan. WWF-Indonesia.
Yu, Q., Xie, J., Huang, M., Chen, C., Qian, D., Qin, J. G., Chen, L., Jia, Y., and Li, E. 2020. Growth and health responses to a long-term pH stress in Pacific white shrimp Litopenaeus vannamei. Aquaculture Reports, 16, 100280.
DOI: http://dx.doi.org/10.20884/1.oa.2025.21.1.1169

This work is licensed under a Creative Commons Attribution 4.0 International License.
Omni-Akuatika by Fisheries and Marine Science Faculty - Jenderal Soedirman University is licensed under the Creative Commons Attribution 4.0 International License.