Efficacy of GP-11 KHV DNA Vaccine in Common Carp (Cyprinus carpio) through Feed by Different Frequency of Administration

Ahmad Beni Rouf, Sri Nuryati, Sukenda Sukenda, Alimuddin Alimuddin

Abstract


GP-11 KHV DNA vaccine is a vaccine that can be used to induce immunity against the KHV virus (Koi herpesvirus). Vaccination through feed is an alternative way of administering vaccines. The study aimed to examine the effect of giving KHV GP-11 DNA vaccine through feed with different frequencies to KHV infection. The frequency of vaccine administration is GP-11 vaccination once a week; GP-11(1x), GP-11 vaccination twice a week; GP-11(2x), GP-11 vaccination three times a week; GP-11(3x), GP-25 vaccinations three times a week; GP-25(3x), negative control (without KHV test) and positive control (KHV tested). The fish were kept for 28 days after vaccination and then continued with the KHV challenge test for 28 days. The weight of carp ranges from 13.82±2.37 g maintained with a density of 15 fish/aquarium. The results showed that vaccine treatment was able to induce an immune response as indicated by the number of white blood cells, lysozyme activity and post-vaccination antibody titer showed a significant effect compared to controls. Likewise, after the challenge test, supported by IFNγ and IgM gene expression parameters after the challenge test showed the highest value of vaccine treatment rather than control. The efficacy of vaccine was showed by RPS value (%) in each vaccine treatment obtained GP-11(1x) value of 44.7±3.7a, GP-11(2x) of 78.9±18.2b, GP-11(3x) 85.6±12.6b and GP-25(3x) 79.5±18.1b. It was concluded that administering the GP-11 vaccine frequency 2 times a week provides protection as strong as giving a vaccine frequency 3 times a week.

Keywords: common carp, DNA vaccine, frequency of administration, koi herpesvirus

Full Text:

PDF

References


Adamek M, Steinhagen D, Irnazarow I, Hikima J, Jung T-S, Aoki T. 2014. Biology and host response to Cyprinid herpesvirus 3 infection in common carp. Developmental and Comparative Immunology. 43(2): 151-159.

Amend DF. 1981. Potency testing of fish vaccines. International Symposium on Fish Biologics: Serodiagnostics and Vaccines. Developments in Biological Standardization. 49:447-454.

Anderson DP, Siwicki AK. 1993. Basic hematology and serology for fish health programs. Paper Presented in Second Symposium on Diseases in Asia Aquaculture "Aquatic Animal Health and The Environmental". Phuket Thailand. 25-29th October 1993.

Anuradha K, Foo HL, Mariana NS, Loh TC, Yusoff K, Hassan MD, Sasan H, Raha AR. 2010. Live recombinant Lactococcus lactis vaccine expressing aerolysin genes D1 and D4 for protection against Aeromonas hydrophila in tilapia (Oreochromis niloticus). Journal of Applied Microbiology. 109(5):1632-1642.

Aoki T, Hikima J, del Castillo CS, Jung TS, Kondo H, Hirono I. 2011. Molecular immunity in the interaction between fish and pathogen for DNA vaccine. Hlm. 253-268. Di dalam: Bondad-Reantaso, M.G., Jones, J.B., Corsin, F. and Aoki, T, editor. Diseases in Asian Aquaculture VII. Fish Health Section, Asian Fisheries Society, Selangor, Malaysia. Hlm. 385.

Aonullah AA, Nuryati S, Alimuddin, Murtini S. 2016. Efficacy of koi herpesvirus DNA vaccine administration by immersion method on Cyprinus carpio field scale culture. Aquaculture Research. 1-8.

Bergmann SM, Lutze P, Schutze H, Fischer U, Dauber M, Fichtner D, Kempter J. 2010. Goldfish (Carassius auratus) is a susceptible species for koi herpesvirus (KHV) but not for KHV disease. Bulletin- European Association of Fish Pathologists. 30:74-84.

Biering E, Salonius K. 2014. DNA vaccines. in: Gudding R, Lillehaug A, Evensen O, editor. Fish Vaccination 1st ed. John Wiley & Sons, Ltd.

Chairunnisa SA, Nuryati S, Alimuddin, Murtini S, Santika A, Yanti DH. 2016. Efficacy of GP-11 KHV DNA vaccine in Cyprinus carpio haematopterus. Journal of Indonesian Aquaculture. 11:31-39.

Chen L, Evensen Ø, Mutoloki S. 2015. IPNV antigen uptake and distribution in Atlantic salmon following oral administration. Viruses. 7(5):2507-2517.

Chen L, Klaric G, Wadsworth S, Jayasinghe S, Kuo T-Y, Evensen Ø, Mutoloki S. 2014. Augmentation of the antibody response of atlantic salmon by oral administration of alginate-encapsulated IPNV Antigens. PLoS ONE. 9(10).

Coban C, Koyama S, Takeshita F, Akira S, Ishii KJ. 2008. Molecular and cellular mechanisms of DNA vaccines. Humam Vaccines. 4(6):453-456.

Cui L-C, Guan X-T, Liu Z-M, Tian C-Y, Xu Y-G. 2015. Recombinant lactobacillus expressing G protein of spring viremia of carp virus (SVCV) combined with ORF81 protein of koi herpesvirus (KHV): a promising way to induce protective immunity against SVCV and KHV infection in cyprinid fish via oral vaccination. Vaccine. 33(27):3092–3099.

Ellis A.E., 1988. Fish Vaccination. Academic Press, New York.

Embregts CWE, Forlenza M. 2016. Oral vaccination of fish: Lessons from humans and veterinary species. Developmental and Comparative Immunology. 64: 118-137.

Gao Y, Pei C, Sun X, Zhang C, Li L, Kong X. 2018. Plasmid pcDNA3.1-s11 constructed based on the S11 segment of grass carp reovirus as DNA vaccine provides immune Protection. Vaccine. 36(25):3613-3621.

Gotesman M, Kattlun J, Bergmann SM, El-Matbouli M. 2013. CyHV-3: The third cyprinid herpesvirus. Diseases of Aquatic Organisms. 105(2):163–174.

Huang LY, Wang KY, Xiao D, Chen DF, Geng Y, Wang J, He Y, Wang EL, Huang, JL, Xiao GY. 2014. Safety and immunogenicity of an oral DNA vaccine encoding Sip of Streptococcus agalactiae from Nile tilapia (Oreochromis niloticus) delivered by live attenuated Salmonella typhimurium. Fish and Shellfish Immunology. 38(1):34-41.

Joosten PH, Engelsma MY, van der Zee MD, Rombout JH. 1997. Induction of oral tolerance in carp (Cyprinus carpio L.) after feeding protein antigens. Veterinary Immunology and Immunopathology. 60(1–2):187–196.

Kole S, Kumari R, Anand D, Kumar S, Sharma R, Tripathi G, Makesh M, Rajendran KV, Bedekar MK. 2018. Nanoconjugation of bicistronic DNA vaccine against Edwardsiella tarda using chitosan nanoparticles: Evaluation of its protective efficacy and immune modulatory effects in Labeo rohita vaccinated by different delivery routes. Vaccine. 36(16): 2155-2165.

Litwack G. 1955. Photometric determination of lysozyme activity. Proceedings for the Society for Experimental Biology and Medicine.89:401-3.

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402–408.

Lu L, Xu H, He Y, Li, J. 2011. Protection of grass carp, Ctenopharyngon idellus (Valenciennes), through oral administration of a subunit vaccine against reovirus. Journal of Fish Disease. 34(12):939-942.

Magnadottir B. 2006. Innate immunity of fish (overview). Fish and Shellfish Immunology. 20:137–51.

Medzhitov R, Jr J.C., 2016. Innate immune recognition and control of adaptive immune responses. Seminars in Immunology. 10(5):351-353.

Munang’andu HM, Mutoloki S, Evensen Ø. 2015. An overview of challenges limiting the design of protective mucosal vaccines for finfish. Review. Frontiers in Immunology. 6:542.

Mutoloki S, Munang’andu HM, Evensen Ø. 2015. Oral vaccination of fish-antigen preparations, uptake, and immune induction. Frontiers in Immunology. 6:519.

Nakanishi T, Toda H, Shibasaki Y, Somamoto T. 2011. Cytotoxic T cells in teleost fish. Developmental and Comparative Immunology. 35(12):1317-1323.

Nur-Nazifah, M., Sabri, M.Y., Siti-Zahrah, A., 2014. Development and efficacy of feed-based recombinant vaccine encoding the cell wall surface anchor family protein of Streptococcus agalactiae against streptococcosis in Oreochromis sp. Fish and Shellfish Immunology. 37(1):193-200.

Nuryati S, Alimuddin, Sukenda, Soejoedono RD, Santika A, Pasaribu FH, Sumantadinata K. 2010. Construction of a DNA Vaccine using glycoprotein gene and its expression towards increasing survival rate of KHV-Infected common carp Cyprinus carpio. Jurnal Natur Indonesia.13: 47–52.

Nuryati S, Khodijah S, Alimuddin, Setiawati M. 2015. Effectiveness of DNA vaccine in feed to koi herpesvirus-infected common carp. Jurnal Kedokteran Hewan. 9:33-37.

Nuryati S, Yulianti, Alimuddin. 2013. Frekuensi dan persistensi vaksin DNA penyandi GP25 yang diberikan melalui pakan pada ikan mas. Jurnal Akuakultur Indonesia. 12(2): 150-157.

Pei C, Gao Y, Sun X, Li L, Kong X. 2019. A developed subunit vaccine based on fiber protein VP56 of grass cap reovirus providing immune protection against grass carp hemorrhagic disease. Fish and Shellfish Immunology. 90:12-19.

Plant KP and Lapatra SE. 2011. Advances in fish vaccine delivery. Developmental and Comparative Immunology. 35:1256–1262.

Rice J, Ottensmeier CH, Stevenson FK. 2008. DNA vaccines: precision tools for activating effective immunity against cancer. Nature Reviews Cancer. 8(2):108-120.

Rombout JHWM, Kiron V. 2014. Mucosal vaccination of fish. Di dalam: Gudding R, Lillehaug A, Evensen O, editor. Fish Vaccination. Oxford: Willey Blackwell. hlm. 56–67.

Seo JY, Chung HJ, Kim TJ. 2013. Codon-optimized expression of fish iridovirus capsid protein in yeast and its application as an oral vaccine candidate. Journal of Fish Disease. 36(9):763-768.

Shin YJ, Kwon TH, Seo JY, Kim TJ. 2013. Oral immunization of fish against iridovirus infection using recombinant antigen produced from rice callus. Vaccine. 31(45):5210-5215.

Siriyappagouder P, Shankar KM, Naveen Kumar BT, Patil R, Byadgi OV. 2014. Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Channa striatus. Fish and Shellfish Immunology, 41(2):581-585.

Skinner LA, LaPatra SE, Adams A, Thompson KD, Balfry SK, McKinley RS. Schulte PM. 2010. Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune respons in Atlantic salmon (Salmo salar L.). Fish and Shellfish Immunology. 28(4):579-586.

Stevenson FK, Ottensmeier CH, Rice J. 2010. DNA vaccines against cancer come of age. Current Opinion in Immunology. 22(2):264-270.

Sunarto A, McColla KA, Crane MSJ, Schat KA, Slobedman B, Barnes AC, Walker PJ. 2014. Characteristics of cyprinid herpesvirus 3 in different phases of infection: Implications for disease transmission and control. Virus Research. 188: 45–53.

Tian JY, Sun BJ, Luo YP, Zhang YG, Nie P. 2009. Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation. Aquaculture. 288(1-2):14-21.

Xue R, Liu L, Cao G, Xu S, Li J, Zou Y. 2013. Oral vaccination of BacFish-vp6 against grass carp reovirus evoking antibody response in grass carp. Fish and Shellfish Immunology. 34(1):348-355.

Zhang C, Zhao Z, Li J, Song K-G, Hao K, Wang J, Wang GX, Zhu B. 2019. Bacterial ghost as delivery vehicles loaded with DNA vaccine induce significant and specific immune responses in common carp against spring viremia of carp virus. Aquaculture. 504:361-368.

Zhu B, Liu GL, Gong YX, Ling F, Wang GX. 2015. Protective immunity of grass carp immunized with DNA vaccine encoding the vp7 gene of grass carp reovirus using carbon nanotubes as a carrier molecule. Fish and Shellfish Immunology. 42(2):325-334.




DOI: http://dx.doi.org/10.20884/1.oa.2020.16.1.768

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

Lisensi Creative Commons

Omni-Akuatika de Fisheries and Marine Science Faculty - Jenderal Soedirman University est mis à disposition selon les termes de la licence Creative Commons Attribution 4.0 International.

Fondé(e) sur une œuvre à www.ojs.omniakuatika.net.
Les autorisations au-delà du champ de cette licence peuvent être obtenues à www.ojs.omniakuatika.net.