Engineering Technology Fish Farming of Snakeheads Channa striat ,Bloch, 1793) Based Feed Vitamin C Increase to Superior Quality Using Microsatellite

Istiyanto Samidjan, Diana Rachmawati


Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the  weight absolute, survival, FCR,  molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism  treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8)  respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.

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Almaniar. S, Tagwa, F. H., Jubaidah, D. 2012. Growth and Seed Viability Snakeheads Fish (Channa striata) during Maintenance with Different Alessandrini, F., Mazzanti, M., Onofri, V., Turchi, C., Tagliabracci, A., 2008. MtDNA Analysis for Genetic Identification of Fo-rensically Important Insects. Forensic Sci Int Genet Suppl Ser 1, 584–585.

Balkhis, A. B. S., Jamsari, A. F. J., Hwai, T. S., Yasin, Z., Azizah, M. N. S. 2011. Cvidence of Geographical Structuring in the Malaysian Snakehead, Channa striata Based on Partial Segment of the CO1 Gene. J Genetics and Molecular Biology 34(3), 520–523.

Bell, J. G., Henderson, R. J., Tocher, D. R., McGhee, F., Dick, J. R., Porter, A., Ct al. 2002. Substituting Fish Oil with Crude Palm Oil in the Diet of Atlantic Salmon (Salmo salar) Affects Muscle Fatty Acid Composition and Hepatic Fatty Acid Metabolism. Journal of Nutrition 132, 222–230.

Bijaksana, U. 2003. Fish Channa striata cork Blkr One of the Potential of Aquaculture Commodity]. [Research Report]. Department of Aquaculture. University of Gastric Fisheries Faculty, Mangkurat. Banjarbaru 40 p

Bijaksana, U. 2006. Preliminary Study of Bio-eco Snakehead Reproduction in Swamp Bangkau South Kalimantan. [Research Report].

National Symposium on Biotechnology in Aquaculture. 2006. Department of Aquaculture and Marine Science Faculty of Fisheries Agriculturel Bogor University and Research Institute of Freshwater Aquaculture Marine and Fisheries Research Agency. July 5, 2006

Bijaksana. 2012. Cvaluation of 17 ß-Estradiol Concentrations in Snakefish (Channa striata Blkr). J Biosciencetiae 9(1), 31–44).

De Graaf, G. J., Galemoni, F., Banzoussi, B., 1990. Succesful Recruitment Control of Nile tilapia, Oreochromis niloticus by the African Catfish, Clarias gariepinus (Burchell.1822) and the African Snakehead, Ophiocephalus obscuris. Hybridization of Tylapia and African Catish FAO.

Directorate General of Fisheries, 1990. Identification and Spread of Some Types of Freshwater Fish Resources in Public Indonesian Waters]. Jakarta.

Effendi, M. I., 1975. Methods of Fisheries Biology. Bogor, Yayasan Dewi Sri.

Gardner, C. J., Snustad, D. P., Simons, M. J. 1991. Prinsiples of Genetics. 8 th Cd. John Wiley & Sons Inc, Toronto-Canada.

Gerking, S. D. 1978. Ccology of Freshwater Fish Production. Blackwell Scientific Publications. Oxford Cngland.

Hadi, S. 2004. Statistics. Volume 2. Andi Offset. Yogyakarta. p. 290.

King, M. 2003. Fisheries Biology, Assessment and Management. Fishing New Books. Blackwell Science. Oxford Cngland.

Haitham G. Abo, E.A, El-Nahas, AF Mahmoud S., Ibrahim, E. M. 2017. Vitamin C modulates the immunotoxic effect of 17 alpha-methyltestosterone in Nile tilapia. J.Biochemistry, P.1-34.

Hariyanti. 2013. Fecundity and Cgg Diameter of Snakehead (Channa striata Bloch, 1793) at Tempe Lake. Journal Science of Fisheries 8(2), 18–24.

Istiyanto, S., Sardiyatmo. 2007. Study of Fish Spawning in the Snakerheads from Rawa Pening Lake, Central of Java]. [Research Report]. Universitas Diponegoro. p. 50.

Istiyanto, S. 2008. Studies on the Relationship of Fish Length and Weight Snakehead (Channa striata) from Rawa Pening Lake Waters]. [Research Report]. FPIK Undip. p. 60.

Istiyanto, S. 2011. Studies on Fish Gonads Maturity Levels in Lake Rawa Pening Semarang District, Central Java. [Research Report]. FPIK Undip. p. 1–50.

Jamsari, A. F. J., Paul, T. M., Azizah, M. N. S., 2011. Isolation and Multiplex Genotyping of Polymorphic Microsatellite DNA Markers in the Snakehead Murrel, Channa striata. J Genetics and Molecular Biology 34(2), 345–347.

King, M. 2003. Fisheries Biology, Assessment and Management. Fishing New Books. Blackwell Science. Oxford Cngland.

Kpogue, D. N. S., Ayonou, G. A., Toko, I. I., Menen, G. A., Floge, C. D. 2013. Influence of Dietary Protein Levels on Growth, Feed Utilization an Carcas Composition of Snakehead, Parachanna obscura (Gunher,1861) Fingerlings. International of Fisheries and Aquaculture 5(5), 71–77.

Marimuthu, K., Haniffa, M. A., Muruganandam, M., Raj, A. J. A. 2001. Low Cost Murrel Seed Production Technique for Fish Farmers. The Iclarm Quarterly 24 Nos 1 & 2 January-June 2001.

Penang Malaysia. Nam, S. 2011. Sustainable Snakehead (Channa striata) Aquaculture Development in Cambodia, in “AquaFish CRSP Air Breathing Fishes Symposium”. In: Stephanie, I., Hillary, C. (Eds). Proceedings of Air Breathing Fishes. Shanghai, China 18 April 2011

Nam, S., Narith, A., Clliott, V. T., Hien T.T., Pomeroy, R. 2011. Sustainable Snakehead (Channa Striata) Aquaculture Development in Cambodia, in “ AquaFish CRSP Air Breathing Fishes Symposium”. In: Stephanie, I., Hillary, C. (Eds.). Proceedings of Air Breathing Fishes Shanghai, China 18 April 2011.

Prosperous, S. 2006a. Feeding Habits Channa striata. Policy DKP: Marine and Fisheries Research Institute October 2006 Cdition of Fisheries and Aquatic Research General. Palembang. [Internet] accessed on May 25th, 2016 from

Prosperous, S. 2006b. Growth Channa striata. Policy DKP: Marine and Fisheries Research November 2006 Cdition of Fisheries and Aquatic Research Institute General. Palembang. [Internet] accessed on May 25th, 2016 from

Prasetiyono, J., Tasliah, Marka. 2004. Microsatellite: A Promising Molecular Markers. Agrobio Bulletin 6(2), 41–47.

Poiko, A., Ram,,A. J., Chong, A., Hashim, R., Olalekan, A. A. 2012. Cstimation of the Tissue Distribution of mRNA Transcripts for Desaturase and Clongase Cnzymes in Channa striata (Bloch, 1793) Fingerlings using PCR Technique, in “4th International Conference on Agriculture and Animal Science IPCBEE”. In: Poiko, A., Ram, A. J. (Eds). IPCBEE vol.47. IACSIT Press, Singapore. DOI: 10.7763/IPCBEE. 2012. V47. 9.

Potts, G. W., Wootton, R. J. 1984. Fish Reproduction : Strategies and Tactics. Academic Press. New York.

Purdom, C. C. 1993. Genetic and Fish Breeding. 1th Cd Chapman and Hall Fish and Fisheries Series 8 Madras India-London. p. 277.

Qiufen, D., Zhidong, P., Gaoshang, M. 2013. The Snakehead Fish from China is Considered as an Cxotic prHybrid Snakehead Fish Farming in China. November/December 2013 AQUA Culture Asia Pacific Magazine 12. p. 3–36.

Rao, K., Podeti, Benarjee, G. 2015. Haemotological Change in Fresh Water Fish, Channa striata Diagnised with the Cpizootic Ulcerative Syndrome (EUS). International Journal of Advanced Biotechnology and Research (IJBR) 6(2), 238–244.

Ram, J. A., Kuah, M. K., Lim, P. S., Kolkovski, S., Chong, A. S. C. 2008. Influence of Dietary HUFA Levels on Reproductive Performance, Tissue Fatty Acid Profile and Desaturase and Clongase mRNAs Cxpression in Female Zebrafish Danio Rerio. Aquaculture 277, 275–281.

Sakhare, V. B. 2015. Fecundity of Air–breathing Fish Channa striata (Bloch) from Waterbodies of Beed Distict, Maharashtra, India. International Journal of Aquaculture 5(18), 1–3.

Seifert, K. A., Samson, R. M., deWaard, J. R., Houbraken, J., Lévesque, C. A., Moncalvo, G. L. S., Ct al. 2007. Prospects for Fungus Identification using CO1 DNA Barcodes, with Penicillium as a Test Case. Proc Natl Acad Sci USA 104, 3901–3906.

Smith, M. A., Poyarkov, J. N. A., Hebert, P. D. N. 2008. CO1 DNA Barcoding Amphibians: Take the Chance, Meet the Challenge. Mol Ccol Resour 8, 235–246.

Suresh, C., Reddy, A. K., Krishna, G., Sharma, R., Chaudhari, A., Sekar, M., Ct al. 2015. Microsatellite DNA Analysis of Giant Freshwater Prawn (Macrobrachium rosenbergii) from India. The Israel Journal of Aquculture Bamidgeh 67, 1070–1077.

Suryono. 1993. Quantitative Genetics on Larval Growth from Bred Broodstock of Freshwater Prawns (Macrobrachium rosenbergii De Man) Marine Science Dept Diponegoro University Semarang in: Fish Genetic and its Application to Aquaculture and Fisheries Management, SEAMEO Biotrop, Bogor Indonesia 52, 119–125.

Weatherley, A. H. 1972. Growth and Ccology of Fish Population. Academic Press. New York.

Yang, Y., Diana, J. S., Shrestha, S. H., Lin, C. K. 2000. Culture of Mixed-sex Nile tilapia with Predatory Snakehead Aquaculture and Aquatic Resources Management.

Yu, L. L., Xiao, Y. K., Zi, N. Y., Jie, K., Shen, M., Li, M. C. 2009. Genetic Diversity and Historical Demography of Chinese Shrimp Feneropenaeus chinensis in Yellow Sea and Bohai Sea Based on Mitochondrial DNA Analysis. Afr J Biotechnol 8, 1193–1202

Zhang, Y. F., Yue, Y. R., Tian, L. X., Liu, Y. J., Wang, A. L., Yang, H. J., Ct al. 2015. Dietary Phosphorus Requirements of Juvenile Hybrid Tilapia (Oreochromis niloticus female x O. aureus male) Fed Fishmeal-Free Practical Diet. The Israel Journal of Aquaculture-Bamidgeh 67, 1179–1193.

Zhang, N., Qiu, Y., Huang, X., Chen, X., Wang, A., Wang, Y. 2012. Microsatellite Marker Development and Characterization in the Spotted Babylon, Babylonia areolata (Link,1807): Detection of Du.


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