SOFT CORAL (Sinularia sp.) EXTRACTS WITH ANTIBACTERIAL ACTIVITY

Wendy Alexander Tanod, Anita Treisya Aristawati, Masteria Yunovilsa putra, Muliadin Muliadin

Abstract


There is a growing need for new antibacterial agents, in particular because many antibiotics are becoming ineffective due to the emergence of resistant bacterial strains. Soft corals of the Genus Sinularia, Family Alcyoniidae, have potential as a source of terpenoid and steroid compounds with antibacterial activity. These corals may vary in external morphology (shape, colour, size).The aim of this research was to identify extracted fractions with high antibacterial activity. Sinularia sp. specimens were extracted, fractionated based on solvent polarity, and tested for antibacterial bioactivity against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Antibacterial activity of the three fractions varied in strength. The dichloromethane fraction showed strong antibacterial activity, inhibiting S. aureus and E. coli growth at a concentration of 1 mg ml-1, while the ethyl acetate and ethanol fractions were effective at 10 mg ml-1 and 100 mg ml-1, respectively.

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References


Afifi, R., Abdel-Nabi, I. M., & El-Shaikh, K. 2016. Antibacterial activity from soft corals of the Red Sea, Saudi Arabia. Journal of Taibah University for Science, 10(6), 887–895.

Ashnagar, a., & Naseri, N. G. 2007. Analysis of Three Penicillin Antibiotics (Ampicillin, Amoxicillin and Cloxacillin) of Several Iranian Pharmaceutical Companies by HPLC. E-Journal of Chemistry, 4(4), 536–545.

Bishara, A., Rudi, A., Benayahu, Y., & Kashman, Y. 2007. Three biscembranoids and their monomeric counterpart cembranoid, a biogenetic diels-alder precursor, from the soft coral Sarcophyton elegans. Journal of Natural Products, 70(12), 1951–1954.

Bloomfield, S. F. 1991. Methods For Assesing Antimicrobial Activity In Mechanism of Action of Chemical Biocides Thesis Study and Explanation. (S. . Denyer & B. W. Hogo, Eds.). London: Blackwell Scientific Publication.

Blunt, J. W., Copp, B. R., Hu, W.-P., Munro, M. H. G., Northcote, P. T., & Prinsep, M. R. 2009. Marine natural products. Natural Product Reports, 26(2), 170–244.

Brannen, A. ., & Davidson, P. . (1993). Antimicrobial in Foods. New York: Marcel Dekker.

Cheng, S.-Y., Huang, K.-J., Wang, S.-K., Wen, Z.-H., Chen, P.-W., & Duh, C.-Y. 2010. Antiviral and anti-inflammatory metabolites from the soft coral Sinularia capillosa. Journal of Natural Products, 73(4), 771–775.

Gan, S. ., Udin, A., & Vincent, H. S. . 1980. Farmakologi Dan Terapi (Edisi Kedua). Jakarta: Fakultas Kedokteran Universitas Indonesia.

Handayani, D., Edrada, R. a., Proksch, P., Wray, V., Witte, L., Van Ofwegen, L., & Kunzmann, a. 1997. New oxygenated sesquiterpenes from the Indonesian soft coral Nephthea chabrolii. Journal of Natural Products, 60(7), 716–718.

Harborne, J. B. 1998. Phytochemical Methods; A Guide to Modern Techniques of Plant Analysis. Journal of Chemical Information and Modeling (Vol. 3). London New York: Chapman and Hall.

Ishii, T., Matsuura, H., Zhan, Z. Q., & Vairappan, C. S. 2009. A New Norsesquiterpenoid from a Bornean Soft Coral Genus Nephthea. Molecules, 14(11), 4591–4596.

Jawetz, Melnick, & Adelberg. 2008. Mikrobiologi Kedokteran. PhD (Edisi Ke-2). Jakarta: EGC.

Jia, R., Guo, Y., Mollo, E., & Cimino, G. 2005. Natural Product Research : Formerly Natural Product Letters Two new 19-oxygenated polyhydroxy steroids from the hainan soft coral Sinularia sp . Natural Product Research, 19(8 December 2005), 789–794.

Lakshmi, V., & Kumar, R. 2009. Metabolites from Sinularia species. Natural Product Research, 23(9), 801–850.

Latifah, Q. A. 2008. Uji Efektifitas Ekstrak Kasar Senyawa Antibakteri Pada Buah Belimbing Wuluh (Averrhoa bilimbi L.) Dengan Variasi Pelarut. Skripsi. Universitas Islam Negeri (UIN) Malang.

Liang, L.-F., Wang, X.-J., Zhang, H.-Y., Liu, H.-L., Li, J., Lan, L.-F., … Guo, Y.-W. 2013. Bioactive polyhydroxylated steroids from the Hainan soft coral Sinularia depressa Tixier-Durivault. Bioorganic & Medicinal Chemistry Letters, 23(5), 1334–1337.

Liang, L. F., & Guo, Y. W. 2013. Terpenes from the soft corals of the genus Sarcophyton: Chemistry and biological activities. Chemistry and Biodiversity, 10(12), 2161–2196.

Madigan, M. ., Martinko, J. ., & Parker, J. 2000. Brock Biology of Microorganisms (9th ed.). New Jersey: Prentice-Hall Inc.

Putra, M. Y., Wibowo, J. T., Murniasih, T., & Rasyid, A. 2016. Evaluation of antibacterial activity from Indonesian marine soft coral Sinularia sp. AIP Conference Proceedings, 1744.

Rachmaniar, R. 1995. Penelitian Produk Alam Laut Skreening Substansi Bioaktif. Laporan. Jakarta.

Radić, N., & Strukelj, B. 2012. Endophytic fungi: the treasure chest of antibacterial substances. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 19(14), 1270–1284.

Rahal, J. J., & Simberkoff, M. S. 1979. Bactericidal and bacteriostatic action of chloramphenicol against meningeal pathogens. Antimicrobial Agents and Chemotherapy, 16(1), 13–18.

Rajaram, S., Ramesh, D., Ramulu, U., Anjum, M., Kumar, P., Murthy, U. S. N., … Venkateswarlu, Y. 2014. Chemical examination of the soft coral Sinularia kavarattiensis and evaluation of anti-microbial activity. Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry, 53(8), 1086–1090.

Rice, L. B. 2008. Federal funding for the study of antimicrobial resistance in nosocomial pathogens: no ESKAPE. The Journal of Infectious Diseases, 197(8), 1079–1081.

Rina. 2011. Substansi Antibakteri dari Ascidian dan Jamur Laut yang Berasosiasi Dengannya. Thesis. Sam Ratulangi University.

Rozirwan, Bengen, D. G., Zamani, N. P., Effendi, H., & Chaidir. 2014. Screening on the potential bioactive compounds of antibacterial activity in soft coral collected from South Bangka Island Waters and Lampung Bay. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 6(2), 1–38.

Sarma, N. S., Krishna, M. S., Pasha, S. G., Rao, T. S. P., Venkateswarlu, Y., & Parameswaran, P. S. 2009. Marine metabolites: The sterols of soft coral. Chemical Reviews, 109(6), 2803–2828.

Shaaban, M., Shaaban, K. A., & Ghani, M. A. 2013. Hurgadacin: a new steroid from Sinularia polydactyla. Steroids, 78(9), 866–873.

Sun, P., Meng, L. Y., Tang, H., Liu, B. S., Li, L., Yi, Y., & Zhang, W. 2012. Sinularosides A and B, bioactive 9,11-secosteroidal glycosides from the South China Sea soft coral Sinularia humilis Ofwegen. Journal of Natural Products, 75(Table 1), 1656–1659.

Tanod, W. A., Mangindaan, R. E. ., & Kapojos, M. 2015. Aktivitas Antimitotik Dari Ekstrak Karang Lunak Genus Sinularia. OmniAkuatika, 11(2), 41–49.

Wang, S.-K., Hsieh, M.-K., & Duh, C.-Y. 2013. New diterpenoids from soft coral Sarcophyton ehrenbergi. Marine Drugs, 11(11), 4318–4327.

Wright, G. D., & Sutherland, A. D. 2007. New strategies for combating multidrug-resistant bacteria. Trends in Molecular Medicine, 13(6), 260-267.

Zhao, M., Yin, J., Jiang, W., Ma, M., Lei, X., Xiang, Z., … Yan, P. 2013. Cytotoxic and antibacterial cembranoids from a South China Sea soft coral, Lobophytum sp. Marine Drugs, 11(4), 1162–1172.




DOI: http://dx.doi.org/10.20884/1.oa.2018.14.1.375

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