Correlation Between Paddle Wheel Operation Time with Microalgae (Nannochloropsis sp.) Growth Rate and Lipid Contents in Open Raceway Ponds

Welmar Olfan Barat, Mujizat Kawaroe, Dietriech G Bengen


Nannochloropsis sp. is one of microalgaes species with high lipid content, so it has great potential to be used as the source of biofuel. One of the microalgae cultivation system in mass scale is open raceway ponds. Problems faced in microalgae cultivation with open raceway ponds system are low productivity and the high production cost, especially high electrical energy for operating paddle wheel machine. This research studies about efficiency and effectiveness level of the cultivation system with open raceway ponds through the analysis of correlation between operational duration of the paddle wheel with growth rate and lipid content from Nannochloropsis sp.. The highest density is 530x106 cell/ml found in treatment of 45min/h. The highest growth rate is 0,45/days Which also found in the treatment of 45min/h but the treatment 45min/h is only able to survive until 12 days. The highest productivity of dry biomass is 0,25 g/L/day found in treatment of 45min/h. Meanwhile, the highest lipid content contained on treatment 15min/h is 5,19%DCW. Dominant fatty acids for all treatments are palmitic acids. The best treatment related to the use of the paddle wheel machine is 15min/h.

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Cassano A. E., Martin C.A., Brandi R.J., Alfano O.M.. 1995. Photoreactor analysis and design: fundamentals and applications. Ind. Eng. Chem. Res., 34, 2155-2201.

Chisti Y. 2007. Biodiesel from Microalgae. Biotechnol Advan. 25: 294-306. doi: 10.1016/j.biotechadv.2007.02.001.

Chiu SY., Kao CY, Tsai MT., Ong SC., Chen CH., Lin CS.. 2009. Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresource Technology. 100 : 833–838.

Gudin C., Chaumont D.. 1991. Cell fragility – The key problem of microalgae mass production in closed photobioreactors. Bioresource Technol 38. P145-151

Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., et al., 2008. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54, 621–639.

Kawaroe M., Prartono T., Sunuddin A., Wulan Sari D., Augustine D.. 2010. Mikroalga Potensi dan Pemanfaatannya untuk Produksi Bio Bahan Bakar. Bogor: IPB Press.

Kawaroe M., Hwangbo J., Agustine D., Putra H. A.. 2015. Comparison of density, specific growth rate, biomass weight, and doubling time of microalgae Nannochloropsis sp. cultivated in Open Raceway Pond and Photobioreactor. AACL BIOFLUX.

Khozin-Goldberg I., Shrestha P., Cohen Z., 2005. Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa BBA-Mol. Cell Biol. Lipids 1738, 63–71.

Klok, A.J., Martens, D.E., Wijffels, R.H., Lamers, P.P., 2013. Simultaneous growth and neutral lipid accumulation in microalgae. Bioresour. Technol. 134, 233–243.

Lavens P., P. Sorgeloos. (1996. Manual on the Production and Use of Live Food for Aquaculture. FAO Fisheries Technical Paper.No. 361.Food and Agriculture Organization of the United Nations. Rome

Mattjik, A., M. Sumertajaya. 2002. Rancangan Percobaan dengan Aplikasi SAS dan Minitab Jilid 1. IPB Press. Institut Pertanian Bogor. Bogor.

Reynolds C. S. 1990. The Ecology of Freshwater Phytoplankton.Cambridge University Press. Cambridge.

Steel, R. G.D., J.H. Torrie. 1989. Prinsip dan Prosedur Statistika. Edisi Kedua. PT. Gramedia. Jakarta.

Vasquez-Duhalt, R., Arredondo-Vega B.Q. 1991. Oil Production From Microalgae Under Saline Stress. Biomassa For Energy and Industry 5 th E.C. Conference, vol 1: Policy, Environment, Production and Harvesting, 1:547-551.

Vincenti W. G., Kruger C. H.. 1965. Introduction to physical gas dynamics, Wiley, New York. 535p.

Wood, A.M., Everroad, R.C., Wingard, L.M., 2005. Measuring growth rates in microalgal cultures. In: Andersen, R.A. (Ed.), Algal Culturing Techniques. Elsevier Academic Press, pp. 269–285.


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