Carrageenan and Garlic Essensial Oil Edible Film as Protective Coating on Catfish Sausage

Eko Nurcahya Dewi, Lukita Purnamayati


The edible film based on carrageenan containing garlic essential oil (GEO) at different concentration (0%; 0,2%; 0,4%; and 0,6%) were added into carrageenan edible film (CEF) then applied on sausage, chilled storage for 10 days. The antioxidative allicin prevention on product determined its chemical characteristics such as TBA, TVBN, Aw, and pH. The addition of garlic essential oil on carrageenan based edible film was not significantly different on tensile strength. CEF with 0,6% of garlic essential oil produced the highest elongation value and the lowest Water Vapor Transmission Rate with the amount of 3,970% and 0,673%; respectively. The catfish sausage coated with 0,4% CEF produced TBA and TVBN value during chilled storage with the amount of 0,870 mg malonaldehyde/kg and 17,223 mgN/100g; respectively and not significantly different to CEF 0,6%. The result indicated that edible film containing garlic essential oil able to retard the oxidation process. Indicated from FTIR test, 0,2%; 0,4%; and 0,6% CEF containing garlic compound illustrated with S-S, N-H, C=O and C-H functional group with the wavelength of 408,91 cm-1, 925,83 cm-1, 1543,05 and 2931,8 cm-1.; respectively.

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Abdou, E. S., & Sorour, M. A. 2014. Preparation and characterization of starch/carrageenan edible films. International Food Research Journal, 21(1), 189–193.

Angeles, T. M., Jesús, P., Rafael, M., & Tania, M. 2016. Evolution of some physicochemical and antioxidant properties of black garlic whole bulbs and peeled cloves. Food Chemistry, 199, 135–139.

Asik, E., & Candogan, K. 2014. Effects of Chitosan Coatings Incorporated with Garlic Oil on Quality Characteristics of Shrimp. Journal of Food Quality, 37, 237–246.

Atares, L., & Chiralt, A. 2016. Trends in Food Science & Technology Essential oils as additives in biodegradable fi lms and coatings for active food packaging. Trends in Food Science & Technology, 48, 51–62.

Chen, C., Liu, C., Cai, J., Zhang, W., Qi, W., Wang, Z., Liu, Z., Yang, Y. 2018. Broad-spectrum antimicrobial activity, chemical composition and mechanism of action of garlic (Allium sativum) extracts. Food Control, 86, 117–125.

Choi, I. S., Cha, H. S., & Lee, Y. S. (2014). Physicochemical and Antioxidant Properties of Black Garlic. Molecules, 19, 16811–16823.

Dewita, & Syahrul. 2015. QualityAssessment of Fish Protein Concentrate from catfish (Pangasius hypopthalmus) During Storage at Room Temperature. IOSR Journal of Environmental Science, Toxicology and Food Technology, 9(9), 20–23.

Dincer, M. T., & Cakli, S. 2015. Textural acceptability of prepared fish sausages by controlling textural indicators. Turkish Journal of Veterinary and Animal Science, 39, 364–368.

Divya, B. J., Suman, B., Venkataswamy, M., & Thyagaraju, K. 2017. A study on phytochemicals, functional groups and mineral composition of Allium sativum (Garlic) cloves. International Journal of Current Pharmaceutical Research, 9(3) : 42-45.

El-sayed, H. S., Chizzola, R., Ramadan, A. A., & Edris, A. E. 2017. Chemical composition and antimicrobial activity of garlic essential oils evaluated in organic solvent, emulsifying, and self-microemulsifying water based delivery systems. Food Chemistry, 221, 196–204.

Fratianni, F., Ombra, M. N., Cozzolino, A., Riccardi, R., Spigno, P., Tremonte, P., Coppola, R., & Nazzaro, F. 2016. and anti-proliferative activities of different endemic Italian varieties of garlic (Allium sativum L.). Journal of Functional Foods, 21, 240–248.

Horita, C. N., Farías-campomanes, A. M., Barbosa, T. S., Esmerino, E. A., Gomes da Cruz, A., Bolini, H. M. A., Meireles, M. M. A., & Pollonio, M. A. R. 2016. The antimicrobial , antioxidant and sensory properties of garlic and its derivatives in Brazilian low-sodium frankfurters along shelf-life. Food Research International, 84, 1–8.

Idakwo, P. Y., Negbenebor, C. A., Badau, M. H., & Gbenyi, D. I. 2016. Total volatile base nitrogen (TVBN) and trimethylamine (TMA) content of “ Bunyi youri ” as influenced by the addition of glucose and clove during storage, 4(5), 81–85.

Indrarti, L., & Indriyati. 2017. Incorporation of citrus essential oils into bacterial cellulose-based edible films and assessment of their physical properties. IOP Conference Series: Earth and Environmental Science, 60, 1–6.

Kakroodi, S., Christianus, A., Tan, C. P., Che Man, Y. B. & Ehteshami, F. 2014. Proximate and fatty acid composition of liver and fatty tissue of patincatfish (Pangasianodon hypophthalmus). Iranian Journal of Fisheries Science, 13(3), 541–549.

Kalem, I. K., Bhat, Z. F., Kumar, S., Noor, S., & Desai, A. 2018. The effects of bioactive edible fi lm containing Terminalia arjuna on the stability of some quality attributes of chevon sausages. Meat Science, 140, 38–43.

Kumar, A., Singh, P., & Danish, M. 2013. Changes in proximate, biochemical and microbiological characteristics of dried Labeo gonius fillets during storage at room temperature. African Journal of Biotechnology, 12(20), 2997–3005.

Masniyom, P. 2011. Deterioration and shelf-life extension of fish and fishery products by modified atmosphere packaging. Songklanakarin J. Sci. Technol., 33(2), 181–192.

Meilisza, N. 2009. Budidaya Ikan Patin di Vietnam : Suatu Kajian Untuk Pengembangan Budidaya Ikan Patin Indonesia. Media Akuakultur, 4(1), 26–31.

Muhamad, N. A., & Mohamad, J. 2012. Fatty acids composition of selected Malaysian fishes. Sains Malaysiana, 41(1), 81–94.

Nagarajan, D., & Kumar, T. R. 2017. Fourier transform infrared spectroscopy analysis of garlic (Allium). International Journal of Zoology Studies, 2(6), 11–14.

Naheed, Z., Cheng, Z., Wu, C., Wen, Y., & Ding, H. 2017. Postharvest Biology and Technology Total polyphenols , total fl avonoids , allicin and antioxidant capacities in garlic scape cultivars during controlled atmosphere storage. Postharvest Biology and Technology, 131, 39–45.

Nisar, T., Wang, Z., Yang, X., Tian, Y., Iqbal, M., & Guo, Y. 2018. International Journal of Biological Macromolecules Characterization of citrus pectin films integrated with clove bud essential oil : Physical , thermal , barrier , antioxidant and antibacterial properties. International Journal of Biological Macromolecules, 106, 670–680.

Noor, S., Bhat, Z. F., Kumar, S., & Mudiyanselage, R. J. 2018. Preservative effect of Asparagus racemosus: A novel additive for bioactive edible films for improved lipid oxidative stability and storage quality of meat products. Meat Science.

Nurilmala, M., Nurhayati, T., Syukur, A. G., Vitner, Y., Agus, S. B., & Budiardi, T. 2015. Evaluation of nutritional and color on Indonesian and imported patin fish (Pangasius sp.,) fillets. Advance Journal of Food Science and Technology, 8(8), 576–582.

Petropoulos, S., Fernandes, Â., Barros, L., Ciric, A., Sokovic, M., & Ferreira, I. C. F. R. 2018. Antimicrobial and antioxidant properties of various Greek garlic genotypes. Food Chemistry, 245, 7–12.

Rauf, N. H., Sulistijowati, R. S., & Harmain, R. M. 2015. Mutu Organoleptik Sosis Ikan Lele yang Disubtitusi dengan Rumput Laut. Jurnal Ilmiah Perikanan Dan Kelautan, 3(3), 125–129.

Reitznerova, A., Sulekova, M., Nagy, J., Marcincak, S., Semjon, B., Certik, M., & Klempova, T. 2017. Lipid Peroxidation Process in Meat and Meat Products : A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric High-Performance Liquid Chromatography. Molecules, 22(1–12).

Soltan, H. R., Ahmed, S. M., & Emam, D. A. 2016. Comparative Antibacterial Activity of Garlic Essential Oil Extracted by Hydro-Distillation and Diethyl Ether Extraction Methods on Four Pathogenic Bacteria. Advances in Plants & Agriculture Research Comparative, 4(2), 1–5.

Soto-valdez, H., Ezquerra-brauer, J. M., Márquez-ríos, E., & Torres-arreola, W. 2015. Effect of previous chilling storage on quality loss in frozen (–20°C ) sierra (Scomberomorus sierra) muscle packed with a low-density polyethylene film containing butylated hydroxytoluene. Food Science and Technology, 35(1), 202–206.

Sung, S., Sin, L. T., Tee, T., Bee, S., Rahmat, A. R., & Rahman, W. A. W. A. 2014. Control of bacteria growth on ready-to-eat beef loaves by antimicrobial plastic packaging incorporated with garlic oil. Food Control, 39, 214–221.

Supardan, M. D., Annisa, Y., Arpi, N., Satriana, & Mustapha, W. A. W. 2016. Cassava Starch Edible Film Incorporated with Lemongrass Oil : Characteristics and Application. International Journal on Advanced Science Engineering Information Technology, 6(2), 216–220.

Suput, D., Lazic, V., Pezo, L., Markov, S., Vastag, Z., Popovic, L., Radulovic, A., Ostojic, S., Zlatanovic, S., & Popovic, S. 2016. Characterization of Starch Edible Films with Different Essential Oils Addition. Pol. J. Food Nutr. Sci, 66(4), 277–285.

Teixeira, B., Marques, A., Pires, C., Ramos, C., Batista, I., Saraiva, J. A., & Nunes, M. L. 2014. Characterization of fi sh protein fi lms incorporated with essential oils of clove, garlic and origanum : Physical, antioxidant and antibacterial properties. LWT - Food Science and Technology, 59, 533–539.

Zeb, A., & Ullah, F. 2016. A Simple Spectrophotometric Method for the Determination of Thiobarbituric Acid Reactive Substances in Fried Fast Foods. Journal of Analytical Methods in Chemistry, 2016, 1–5.

Zzaman, W., Suseno, S. H., Nadiah, W. A., & Tajul, A. Y. 2014. Fatty Acid Profile and Antioxidant Capacity of Muscle and by Product Oil from Selected Fresh Water Fish. Food Science and Technology, 2(3), 41–46.


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