Cadmium Induces the Activation, Concentration, and Expression of MTF-1 Protein As a Biomolecular Response of Sea Urchins Deadema setosum

Dominggus Rumahlatu

Abstract


Heavy metal cadmium has toxic effects in the bodies of living organisms.However, the bodies of living organisms have the ability to regulate a wide range of toxic entering the body by using biomolecular response that aims at maintaining cell homeostasis. In this research an induction of cadmium in sea urchins Deadema setosum was carried out. Cadmium was induced in 6 levels of concentration in six treatment groups of D. setosum. Each group consisted of 7 individuals.The examination of MTF-1 protein activation was carried out by immunohistochemical method, the measurement of MTF-1 protein concentration was carried out by the indirect ELISA method (Enzyme Linked Imunoasssay) and the examination of MTF-1 protein expression was carried out by western blotting method. The data were analyzed using descriptive analysis and One Way ANOVA followed by Duncan test 0.05 to determine the mean difference of MTF-1 protein concentration of D. setosum. The research results showed that cadmium induction activated MTF-1 characterized by the condition of liver cells of D. setosum which was brown.The higher the exposure of cadmium concentration on D. setosum, the higher the concentration of MTF-1 expressed

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References


Adams, T.K., Saydam, N., Steiner, F., Schaffner, W., Freedman, J.H. 2002. Activation of gen expression by metal-responsive signal transduction pathway. Enviromental Health Perspectives 110(Suppl 5): 813–817.

Ali, C., Réda, D.M., Rachid, R., Houria, B. 2009. Cadmium Induced Changes in Metabolic Function of Mitochondrial Isolated from Potato Tissue (Solanum tuberosum L.). American Journal of Biochemistry and Biotechnology 5(1): 35-39.

Almeida, J.A., Barreto, R.E., Novelli, L.B., Castro, F.J., Moron, S.E. 2009. Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination. Neotropical Ichtyology 7(1): 103-108.

Balamurugan, K., Hua, H., Georgiev, O.,Schaffner, W., 2009. Mercury and Cadmium trigger expression of the copper importer Ctr1B, which enables Drosophila to thrive on heavy metal-loaded food. Biol. Chem. 390(2), 109-113.

Choudhary, M., Jetley, U.K., Khan, M.A., Zutshi, S.,Fatma, T. 2006. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotoxicol Environ Saf 66(2): 204-209.

Dailianis, S., Kaloyianni, M. 2004. Cadmium induces both pyruvate kinase and Na+/H+ exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cellss of Mytilus galloprovincialis (L.). The Journal of Experimental Biology 207: 1665-1674.

Formigari, A., Alberton, P., Cantale, V., De Nadal, V., Feltrin, M., Ferronato, S., Santon, A., Schiaovon, L.,Irato, P., 2008. Relationship between metal transcription fakctor-1 and Zinc in resistance to metals producing free radicals. Current Chemical Biology 2: 256-266.

Gunther, V., Lindert, U., Schaffner, W. 2012. The taste of heavy metals: Gene regulation by MTF-1. Biochimica et Biophysica Acta 1823(9): 1416-1425.

Gunes, C., Heuchel, R., Georgiev, O.,Muller, K-H., Lichtlen, P.,Bluthmann, H., Marino, S., Aguzzi, A., Schaffner, W. 1998. Embryonic lethality and liver degeneration in mice lacking the metal responsive transcriptional activator MTF-1. The EMBO Journal 17(10): 2846-2854.

Gzyl, J., Rymer, K., Gwozdz, E.A. 2009. Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L). Acta Biochemica Polonica 56(4): 723-727.

Janssens, T.K.S., Roelofs, D., van Straalen, N.M. 2009. Molecular mechanisms of heavy metal tolerance and evolution in Invertebrates. Insect Science 16, 3-18.

Jiang, H., Fu, K., Andrews. 2004. Gene-and cell-type-spesific effects of signal transduction cascades on metal-regulated gene transcription appear to be independent changes in the phosphorylation of metal response element binding transcription factor-1. Biochem Journal 382: 33-41.

Jonak, C., Nakagami, H., Hirt, H. 2004. Heavy metal stress. Activation mitogen-activated protein kinase pathways by copper and cadmium. Plant Physiology 136: 3276-3283.

Kimura, T., Itoh, N. 2008. Function of metallothionein in gen expression and singnal transduction: Newly found protective role of metallothionein. Journal of Health Science 54(3): 251-260.

Kimura, T., Itoh, N., Andrews, G.K. 2009. Mehachanismes of heavy metal sensing by metal response elemen binding transcription factor-1. Journal of Health Science 55(4): 484-494.

Klaassen, C.D., Liu, J., Choudhuri, S. 1999. Metallothionein: an intracellular protein to protect against cadmium toxicity. Annu Rev Pharmacol Toxicol. 39: 267-294.

Langmade, S.J., Ravindra, R., Daniels, P.J., Andrews. 2000. The regulation factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. The Journal of Biological Chemistry 275(44): 34083-34089.

Lequin, R.M. 2005. Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). Clinical Chemistry 51(12): 2415–2418.

Li, Yong., Kimura, T., Laity, J.H., Andrews, G.K. 2006. The zinc-sensing mechanisms of mouse MTF-1 involves linker peptides between the zinc finger. Molecular and cellular Biology 26: 5580-5587.

Lichtlen, P., Schaffner, W. 2001. The “Metal transcription factor” MTF-1: Biological Facts and Medical Implications. SWISS MED WKLY 131: 647-652.

Lindert, U., Cramer, M., Meuli, M., Georgiev, O., Schaffner, W. 2009. Metal-responsive transcription factor 1 (MTF-1) activity is regulated by a nonconventional nuclear localization signal and a metal-responsive transactivation domain. Molecular and cellular Biology 29(23): 6283-6293.

Martin, P., Pognonec, P. 2009. ERK dan cell death: cadmium toxicity, sustained ERK activation and cell death. FEBS Journal 277: 39-46.

Nielsen, A.E., Bohr, A., Penkowa M. 2006.The balance between life and death of cell: role of metallothioneins. Biomarker Insights 1: 99-111.

Ohta, H., Yamauchi, Y., Nakakita, M., Tanaka, H., Asami, S., Seki, Y., Yoshikawa, H. 2000. Relationship between Renal Dysfunction and Bone Metabolism Disorder in Male Rats after Long-Term Oral Quantitative Cadmium Administration. Industrial Healthol 38: 339–355.

Olive, L., Sisk, J., Bressler, J. 2001. Involvement of DMT1 in uptake of Cd in MDCK cells” role of protein kinase C. Am. J. Physiol Cell 281: 793-800.

Radtke, F., Georgiev, O., Muller, H.P., Brugnera, E., Schaffener, W. 1995. Fuctional domains of the heavy metal-responsive transcription regulator MTF-1. Nucleic Acid Research 23(12): 2277-2286.

Rico, L.G., Felix, C.F., Burguenso, R.R., Marini, M.J. 2002. Determination of cadmium and zinc and its relathionship to metalloghionein level in swine kidney. Rev. In. Contant Ambient 18(4): 157-162.

Rumahlatu, D., Corebima, A.D., Amin, M., Rochman, F. 2012. The Effect of Cadmium on Metallothionein Protein Expression of Deadema setosum (Echinoidea; Echinodermata). Jurnal Penelitian Perikanan [Fishery Research Journal] 1(1): 26-35.

Sakulsak, N. 2012. Metallothionein: An overview on its metal homeostatic regulation in mammals. Int. J. Morphol 30(3): 1007-1012.

Smiri, M., Chaoui, A., Ferjani, E.E. 2010. Interaction between heacy metals and thiol-linked redox reactions in germination. Pakistan Journal of Biological Sciences 13(18): 877-883.

Saydam, N., Adams, T.K., Steiner, F., Schaffner, W.,Freedman, J.H. 2002. Regualtion of metallothionein transcription by the metal-responsive transcription factor MTF-1. The Journal of Biological Chemistry 277 (23): 20438–20445.

Waelput, W., Broekaert, D., Vandekerckhove, J., Broukaert, P., Tavernier, J., Libert, C. 2001. A mediator role for metallothionein in Tumor Necrosis Factor-induced lethal shock. J. Exp. Med. 9(11): 1617-1624.

Wimmer, U., Wang, Y., Georgiev, O., Schaffner, W. 2005. Two Major Branches of Anti-Cadmium defense in the mouse: MTF-1/ Metallothioneins and glutathione. Nucleic Acid Research 33(18): 5715-5727.




DOI: http://dx.doi.org/10.20884/1.oa.2017.13.2.290

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